The drug discovery and development process use as target of new drug. This process transform some techniques such as screening, optimization, characterization, validation, synthesis. Target based drug design is more important, long-term, successful process. Which is use as high Throughput screening (HTS). Target is synthesized by combination of chemistry. All type of chemistry use in new drug discovery such as analytical chemistry, computational chemistry, organic chemistry, inorganic chemistry, medicinal chemistry, physical chemistry, biochemistry etc. Analytical chemistry in pharmaceutical drug development providing assurance of the quality, safety and efficacy of new drug. Analytical chemistry play and important in developing a new drug. HPLC is most important Analytical process in pharmaceutical industry, other techniques are also used. It’s used as investigate polymorphism in new drug. All drugs are manufacturing under GMP i.e Good manufacturing practices. 50% Analytical chemistry used in drug discovery and development. Analytical method and instrumentation is used in the preclinical testing, toxicity testing and quality control. Commonly used as purification chemistry.