Vol. 4, Issue 2, Part B (2022)

In neurobehavioral research, animal model has played a crucial role in yielding experimental data as well as, in the development of new insights. Researchers are always trying to develop novel animal models to understand fundamental features of physiological, behavioral and psychological disorders. Zebra fish (Danio rerio) is now considered globally as a new and useful model in biomedical research. The objective of the present study was to assess behavioral changes in adult zebra fish after acute exposure to different pharmacological and herbal compound. Adult zebra fish of 4-5-months-old were exposed to different concentrations of unknown test drug with the standard for 5 min. The test was conducted separately for each drug concentration as well as control. Behavioral activity parameters viz. novel tank test and light and dark transition test were recorded for each animal during the exposure period. Results: Zebra fish exposed to Test drug (Calycophyllum spruceanum) showed change in behavioral activity with significant to the standard drug caffeine. In view of the above findings, these results suggested that exposure of adult zebra fish with drug produce the expected changes in the behavior like stress and anxiety; therefore, adult zebra fish can be used an alternative approach for the assessment of new chemical entities for their effect on behavioral activity.