Vol. 7, Issue 1, Part A (2025)

Collagen model peptides, (Xaa-Yaa-Gly)₁₀ have been used to understand the propensity of the amino acid in Xaa nad Yaa positions. Five membered cyclic imino acid, proline (Pro), have found to be most investigated model. In this investigation Pipecolic acid (Pip), a six member cyclic imino acid and a higher homologue of Pro has been used for sysnthesis of (Pro-Pip-Gly)₁₀, (Pip-Pro-Gly)₁₀, and (Pip-Pip-Gly)₁₀ have been synthesized, characterised and compared with (Pro-Pro-Gly)₁₀ for their physical property such as solubility. Their triple helix formation characteristics at 4 °C has been investigated by circular dichroism (CD) spectroscopy. It has been found that Pip has higher propensity for Yaa position compared to Xaa position in triple helix conformations.