Vol. 7, Issue 2, Part A (2025)

In the present work, a stability-indicating reverse phase HPLC (RP-HPLC) technique using an AD HOC Prudent C18 (125 mm x 4.6 mm; 5µ) P/N:P18-12540-5 column was developed and subsequently validated for the detection of Timolol Maleate and Brinzolamide simultaneously. ICH guidelines were followed for the method's validation. All system suitability parameters were determined to be within the intended ranges. This method was found to be specific, precise, linear, accurate and robust. Forced degradation studies were carried out under various circumstances. The drug molecules were not impacted during stress application in thermal and acidic conditions. This method will be beneficial for qualitative as well quantitative determination of Timolol and Brinzolamide used in combination in process and final formulation.